Hemolytic reaction of normal fresh chicken serum on sheep erythroytes was studied and the following experimental results were obtained and summarized.
1. Chicken sera, 258 (78%) out of 344 samples skewed hemolytic activity on sheep erythrocytes.
2. Distribution of a different hemolytic titer of chicken sera was not dependent to sex and age difference of test chicken.
3. Hemolytic activity of serum component obtained from normal fresh chicken was heat inactivated at 56℃. 30 minutes heating.
4. The mast enhanced hemolytic activity of chicken serum on sheep erythrocytes was observed at the incubation temperature of 46℃.
5. The most effective pH for the hemolytic reaction of chicken serum on sheep erythrocytes was observed at 7.0, and pH 6.0 or 8.5 resulted less or no hemolysis.
6. Hemolytic reaction of chicken serum and sheep erythrocytes required Mg^(++) and Ca^(++) ions as co-factor, and the former was required more compared to the latter.
7. Hemolytic activity of chicken serum was observed in ChC 2, 4 fraction but not in ChC 1, 3, ChC 3, 4, ChC 1, 2, 4 and ChC 1, 2, 3 fractions.
8. In electron micrography, morphological changes of sheep erythrocyte membrane by normal chicken serum was similar to that of immune hemolysis: that was, the hemolytic hole was circular and it was surrounded with a white ring.
9. Electron micrography of morphological changes on sheep erythrocyte membrane indicated that the size of hemolytic hole and white ring were functional to the chicken serum concentration used and reaction time.
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